"Vacuum soft lithography to direct neuronal polarization"

J. Tanner Nevill: Alexander Mo, Branden J. Cord, Theo D. Palmer, Mu-ming Poo, Luke P. Lee and Sarah C. Heilshorn; Soft Matter, 02/15/11.

Additional Authors: Alexander Mo, Branden J. Cord, Theo D. Palmer, Mu-ming Poo, Luke P. Lee and Sarah C. Heilshorn

Abstract:

The ability to coat surfaces with pre-determined patterns of biomolecules by soft lithographyhas found use in areas ranging from fundamental biology to translational medicine, such as tissue engineering and diagnostics. However, existing surface patterningtechniques (e.g.,microcontact printing and traditionallithography) are unable to pattern several biomolecules in a single step. Here we introduce a simple method to simultaneously pattern multiple biomolecules in complex two-dimensional configurations onto substrates with better than 2 ┬Ám resolution. This protocol, termed vacuumsoft lithography, utilized below ambient pressures temporarily stored within a removable microfluidic template to expose specific regions of a substrate to multiple biochemical solutions. We demonstrate the utility of this vacuumsoft lithography technique by fabricating a multi-component array that directs the adhesion, polarization, andneurite guidance of primary hippocampalneurons.